PRELIMINARY QUALITATIVE, QUANTITATIVE SCREENING OF DIFFERENT EXTRACTS OF ADANSONIA DIGITATA L AND ISOLATION AND CHARACTERIZATION OF KAEMPFEROL 3-O RUTINOSIDE FROM ADANSONIA DIGITATA L

Abstract

Adansonia digitata L belongs to family Malvaceae Known as Baobab is found in Southern Africa, Botsana, Namibia, Mozambique and other tropical African countries where it is suitable. The tree has grown in various parts of India especially in Bombay, Andhra, Bihar, Pradesh, Gujarat, and Coromandel Coastand Ceylon. The baobab fruit it is increasingly known for its nutritional and polyphenol content. In this investigation Preliminary Qualitative, Quantitative screening of Different extracts of Adansonia digitata L was carried. Ethanolic Fruit extract of Adansonia digitata L (EFAD) shows better results as compared to other Extracts especially for Total flavanoid Content (TFC) and Total Phenolic Content (TPC).In this study,the flavonoids were extracted from Ethanolic Fruit extract of Adansonia digitata L (EFAD). The presence of kaempferol 3-O-rutinoside was inferred from LC/MS/MS data for crude fruit extracts and compared with Standard Sample. Their structures were identified using UV, LC-MS, IR and 1H NMR, 13C NMR, HPTLC, HPLC spectroscopy. The compound was eventually identified as kaempferol 3-O-rutinoside.