STUDIES ON SYNTHESIS AND CHARACTERISATION OF AEGLE MARMELOS (BAEL LEAVES) SUPERFINE NANO POWDER USING MODERN SCIENTIFIC TOOLS, AN ECO-FRIENDLY APPROACH AND ITS CYTOTOXICITY EVALUATION.

Abstract

The present research focuses on the eco-friendly synthesis of Aegle marmelos leaf superfine nanoparticles using high-energy ball mills method. The coarsely ground powder labelled as 0hr was milled for 2hrs. 4hrs. and 6hrs through high energy ball mill. Morphological study showed high agglomeration in all three milled samples confirmed through SEM. The particle size was evaluated using TEM analysis showed that the milled nanopowder is approximately 17.89 nm. The Zeta potential found to increase with milling hour from -12.52 mV and -10.84 mV, showing the stability increases with milling. An amorphous structure showed by XRD measurement, despite the nanoparticles' size changes as a result of milling time. Uv-Visible-NIR measurement indicates the maximal absorbance found almost the same at 400 nm and somewhat lower at 280 nm and further band gap was evaluated and found in the range of 2.25 eV and 1.75 eV. The functional group were remains unchanged during milling, which was analysed by FTIR. The FTIR and UV-vis-NIR spectra demonstrate that milling did not affect on the materials’ internal covalent nature, but surface reactivity was determined to have risen, that was further supported through a SEM results. Using the MTT assay, the produced superfine powder's impact on mice splenocyte cell line was evaluated basically a colorimetric assay. The test reveals that the altered structure of the superfine powder has resulted in decreased cell toxicity and higher cell viability. The current study supports the idea that a change in crystalline size does not always mean that the phytochemicals contained in the sample have changed, but that their physiochemical properties have increased instead, which is useful for various applications in biomedical applications.