SYNTHESIS OF MAGNESIUM NANOPARTICLE AND NANOCOMPOSITE IN EXTRA-CELLULAR FILTRATES FROM MICROBIAL CULTURES FOR WASTE DEGRADATION

Abstract

Magnesium oxide nanoparticles are currently used for different environmental safety applications because they exhibit high ionic properties, high stability, biocompatibility, and high-contaminant adsorption; they are also safe and cost effective. Conventional methods of Magnesium oxide nanoparticle synthesis are known to have strong deleterious impact on the environment thus; microbial biosynthesis method gained more attention. This study synthesized magnesium nanoparticles from beneficial oligotrophic microbial cultures, and tested on solid waste degradation. Microbes were isolated from the soil, roots of soybeans, and ripe pineapple employing routine microbiology techniques, and assessed for their degradative capacities. Top three isolates with the best degradative index were made the choice isolates for nanoparticle production, and their identities were confirmed using molecular sequencing. Magnesium nanoparticles were synthesized from a blend of the choice isolates, and characterized using ultraviolet-visible (UV-VIS) spectrophotometric analysis, Fourier transforms (FTIR) infra-red, X-ray diffraction (XRD) investigation, and scanning electron microscopic (SEM). Synthesized nanoparticles were checked for their waste degradation potential through composting. Enterobacter sp., Bacillus thuringiensis and Candida tropicalis were the effective organisms identified using molecular sequencing. UV-VIS spectral profile of the microbially synthesized magnesium nanocomposite showed highest absorption peak of 1.08 was found at 500 nm, FTIR profile showed the highest broad and intense absorption peak was a wavenumber frequency region of 3715.104 cm-1, X-ray diffraction showed diffraction-intense peaks in the spectrum of two-degree theta shift data ranging from 2° to 70°, and scanning electron micrograph showed that the MgO nanocomposite was a dispersed, aggregated nano spherical sheet in shape. All the composting units had fine homogenous materials in texture, dark brown to black in colour, slightly foul to earthy smell in odour, 12.0, 7.2, 7.2, 5.5 cm in compactness (height) and 4.067, 2.595, 3.318, 2.648 kg in mass reduction for control, effective microorganisms, magnesium nanocomposite and consortium respectively, after the composting period

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Issue

Vol. 97 No. 1 (2026)