EVALUATION OF CYNARA CARDUNCULUS ON MCF-7 BREAST CANCER CELL LINE: CYTOTOXIC, APOPTOTIC, AND OXIDATIVE STRESS EFFECTS
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Keywords
Evaluation, Cynara Cardunculus, MCF-7 Breast Cancer Cell Line, Cytotoxic, Apoptotic, Oxidative Stress Effects.
Abstract
Background: Breast cancer remains a major global health concern, necessitating alternative therapeutic approaches due to the limitations of traditional chemotherapy. Cynara cardunculus (artichoke), a medicinal plant known for its antioxidant and anti-inflammatory properties, has gained interest for its potential anticancer effects. Objective: This study aimed to evaluate the cytotoxic, apoptotic, and oxidative stress-inducing effects of C. cardunculus extract on the MCF-7 breast cancer cell line. Methods: MCF-7 cells were treated with various concentrations of C. cardunculus extract for 24, 48, and 72 hours. Cell viability (MTT assay), apoptosis (Annexin V/PI, DNA fragmentation), ROS generation (DCF-DA), and morphological alterations (microscopy) were assessed. Results: The extract significantly reduced cell viability in a dose- and time-dependent manner, induced apoptosis, increased ROS production, and caused morphological changes. Conclusion: Cynara cardunculus exhibits promising anticancer activity and may serve as a potential plant-based therapy for breast cancer pending further molecular and in vivo validation. Index Terms- About four key words or phrases in alphabetical order, separated by commas. Keywords are used to retrieve documents in an information system such as an online journal or a search engine. (Mention 4-5 keywords)
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