STABILITY INDICATING RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF FAVIPIRAVIR IN BULK AND PHARMACEUTICAL DOSAGE FORM.

Abstract

In this work, a new sensitive and accurate high-performance liquid chromatographic method for simultaneous determination of Favipiravir used for the treatment of corona disease was developed and validated. Inertsil ODS-3V C18 (4.6 mm X 100mm, 3.0 µm) column used for the chromatographic separation. Isocratic elution of mobile phase consisting 10 mM potassium dihydrogen phosphate (pH 4.0): Acetonitrile (90:10% v/v) was used for the separation. UV detector was used and detection wavelength was performed at 229 nm. The method was linear over the concentration range of 10 to 100 µg/mL for Favipiravir. The proposed method was validated and successfully applied for commercial dosage form. Method performed was highly accurate, precise and robust. Stability study was performed on different stress condition. Maximum degradation was achieved in alkali degradation.