QUALITATIVE AND QUANTITATIVE DETERMINATION OF WITHAFERIN-A FROM WITHANIASOMNIFERA BY ANALYTICAL METHOD HPTLC
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Keywords
Withaniasomnifera, HPTLC, Withaferin-A, Qualitative determination, Quantitative determination, Soxhlet extraction.
Abstract
Withaferin-A, an exceptionally oxygenated steroidal lactone found in the medicinal plant Withaniasomnifera and this plant belong to the Solanaceae family, is a major component of the withanolide group. Withaferin A have diverse functions including cytotoxic activity against cancer cell.The current study design to aim determination of the qualitatively and quantitatively presence of withaferin A from root powder extract of Withaniasomnifera. Withaniasomnifera root powered was extracted by using soxhlet extraction apparatus and obtained thick pest (extract) by using rota vapour. The extract was analysed by high performance thin layer chromatography (HPTLC). As a solvent methanol was used. Total three tracks were used to run the sample (extract of root power of Withaniasomnifera).Anti tumor compound withaferin A was obtained in the form of band qualitatively and area quantitavely.It is evident from the performed experiments that withaferin A is a major constituent of extract of root power of Withaniasomnifera.
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